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anti-human cd105 apc  (Thermo Fisher)


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    Structured Review

    Thermo Fisher anti-human cd105 apc
    Anti Human Cd105 Apc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti-human cd105 apc/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    anti-human cd105 apc - by Bioz Stars, 2026-06
    90/100 stars

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    Characteristics of adipose-derived stem cells. (a) Positive expression for CD90 and <t>CD105</t> and negative expression for CD19 and CD34. (b), (c) Differentiation of ADSCs into adipocytes and osteoblasts. After adipogenic differentiation, newly differentiated adipocytes had lipid droplets identified by Oil Red O staining. Osteogenic differentiation was confirmed by Alizarin Red S staining. ADSCs: adipose-derived stem cells; CD: cluster of differentiation.
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    antibodies against cd73 apc, cd105 pe, cd45 fitc, and cd34 apc  (Thermo Fisher)
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    Characterization of IFN‐γ‐iMSCs and IFN‐γ‐iMSC‐EVs. (a) Flow cytometry analysis of IFN‐γ‐iMSCs. The reactivities of IFN‐γ‐iMSCs against positive (CD105, CD73 and CD90) or negative (CD45, CD31 and <t>CD34)</t> markers of MSCs were evaluated. (b) Representative image of IDO1 protein expression in iMSCs and IFN‐γ‐iMSCs by immunocytochemistry analysis. Green and blue denote cells stained with anti‐IDO1 antibody and DAPI, respectively. (c) Immunoblot analysis of IDO1 protein levels in iMSCs and IFN‐γ‐iMSCs. n = 3. Data are presented as mean ± SE. ** p < 0.01 versus Control. (d) Scheme of the IFN‐γ‐iMSC‐EVs manufacturing. IFN‐γ‐iMSC‐EVs were isolated from the IFN‐γ‐iMSCs culture medium using ultracentrifugation. (e) Morphology of IFN‐γ‐iMSC‐EVs under cryo‐TEM. Scale bar = 200 nm. (f) Size distribution of IFN‐γ‐iMSC‐EVs shown by NTA. The average size is 134.6 nm. (g) Western blot analyses for markers of extracellular vesicles (CD9 and CD63) or cellular organelles (Cytochrome C and Histone H3) in IFN‐γ‐iMSCs and IFN‐γ‐iMSC‐EVs. (h) Expression analysis of EV markers (CD63 and CD81) in IFN‐γ‐iMSC‐EVs by flow cytometry.
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    antibodies against cd73 apc, cd105 pe, cd45 fitc and cd34 apc  (Thermo Fisher)
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    Characterization of IFN‐γ‐iMSCs and IFN‐γ‐iMSC‐EVs. (a) Flow cytometry analysis of IFN‐γ‐iMSCs. The reactivities of IFN‐γ‐iMSCs against positive (CD105, CD73 and CD90) or negative (CD45, CD31 and <t>CD34)</t> markers of MSCs were evaluated. (b) Representative image of IDO1 protein expression in iMSCs and IFN‐γ‐iMSCs by immunocytochemistry analysis. Green and blue denote cells stained with anti‐IDO1 antibody and DAPI, respectively. (c) Immunoblot analysis of IDO1 protein levels in iMSCs and IFN‐γ‐iMSCs. n = 3. Data are presented as mean ± SE. ** p < 0.01 versus Control. (d) Scheme of the IFN‐γ‐iMSC‐EVs manufacturing. IFN‐γ‐iMSC‐EVs were isolated from the IFN‐γ‐iMSCs culture medium using ultracentrifugation. (e) Morphology of IFN‐γ‐iMSC‐EVs under cryo‐TEM. Scale bar = 200 nm. (f) Size distribution of IFN‐γ‐iMSC‐EVs shown by NTA. The average size is 134.6 nm. (g) Western blot analyses for markers of extracellular vesicles (CD9 and CD63) or cellular organelles (Cytochrome C and Histone H3) in IFN‐γ‐iMSCs and IFN‐γ‐iMSC‐EVs. (h) Expression analysis of EV markers (CD63 and CD81) in IFN‐γ‐iMSC‐EVs by flow cytometry.
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    Characteristics of adipose-derived stem cells. (a) Positive expression for CD90 and CD105 and negative expression for CD19 and CD34. (b), (c) Differentiation of ADSCs into adipocytes and osteoblasts. After adipogenic differentiation, newly differentiated adipocytes had lipid droplets identified by Oil Red O staining. Osteogenic differentiation was confirmed by Alizarin Red S staining. ADSCs: adipose-derived stem cells; CD: cluster of differentiation.

    Journal: Cell Transplantation

    Article Title: Adipose-derived stem cells inhibit dendritic cell migration by secreting tumor necrosis factor-α-stimulated gene 6 to improve the allogeneic skin transplantation survival rate in mice

    doi: 10.1177/09636897251376125

    Figure Lengend Snippet: Characteristics of adipose-derived stem cells. (a) Positive expression for CD90 and CD105 and negative expression for CD19 and CD34. (b), (c) Differentiation of ADSCs into adipocytes and osteoblasts. After adipogenic differentiation, newly differentiated adipocytes had lipid droplets identified by Oil Red O staining. Osteogenic differentiation was confirmed by Alizarin Red S staining. ADSCs: adipose-derived stem cells; CD: cluster of differentiation.

    Article Snippet: Passage 3 cells were stained with antibodies against CD90 (E-AB-F1283C) (Elabscience, Wuhan, China), CD105 (E-AB-F1233UE) (Elabscience, Wuhan, China), CD19 (E-AB-F1004E) (Elabscience, Wuhan, China), and CD34 (E-AB-F1284D) (Elabscience, Wuhan, China).

    Techniques: Derivative Assay, Expressing, Staining

    Characterization of IFN‐γ‐iMSCs and IFN‐γ‐iMSC‐EVs. (a) Flow cytometry analysis of IFN‐γ‐iMSCs. The reactivities of IFN‐γ‐iMSCs against positive (CD105, CD73 and CD90) or negative (CD45, CD31 and CD34) markers of MSCs were evaluated. (b) Representative image of IDO1 protein expression in iMSCs and IFN‐γ‐iMSCs by immunocytochemistry analysis. Green and blue denote cells stained with anti‐IDO1 antibody and DAPI, respectively. (c) Immunoblot analysis of IDO1 protein levels in iMSCs and IFN‐γ‐iMSCs. n = 3. Data are presented as mean ± SE. ** p < 0.01 versus Control. (d) Scheme of the IFN‐γ‐iMSC‐EVs manufacturing. IFN‐γ‐iMSC‐EVs were isolated from the IFN‐γ‐iMSCs culture medium using ultracentrifugation. (e) Morphology of IFN‐γ‐iMSC‐EVs under cryo‐TEM. Scale bar = 200 nm. (f) Size distribution of IFN‐γ‐iMSC‐EVs shown by NTA. The average size is 134.6 nm. (g) Western blot analyses for markers of extracellular vesicles (CD9 and CD63) or cellular organelles (Cytochrome C and Histone H3) in IFN‐γ‐iMSCs and IFN‐γ‐iMSC‐EVs. (h) Expression analysis of EV markers (CD63 and CD81) in IFN‐γ‐iMSC‐EVs by flow cytometry.

    Journal: Journal of Extracellular Biology

    Article Title: iMSC‐Derived Extracellular Vesicles Improve Atopic Dermatitis by Augmenting Skin Barrier Integrity and Inhibiting Inflammation, Pruritus and Th2 Immune Responses

    doi: 10.1002/jex2.70067

    Figure Lengend Snippet: Characterization of IFN‐γ‐iMSCs and IFN‐γ‐iMSC‐EVs. (a) Flow cytometry analysis of IFN‐γ‐iMSCs. The reactivities of IFN‐γ‐iMSCs against positive (CD105, CD73 and CD90) or negative (CD45, CD31 and CD34) markers of MSCs were evaluated. (b) Representative image of IDO1 protein expression in iMSCs and IFN‐γ‐iMSCs by immunocytochemistry analysis. Green and blue denote cells stained with anti‐IDO1 antibody and DAPI, respectively. (c) Immunoblot analysis of IDO1 protein levels in iMSCs and IFN‐γ‐iMSCs. n = 3. Data are presented as mean ± SE. ** p < 0.01 versus Control. (d) Scheme of the IFN‐γ‐iMSC‐EVs manufacturing. IFN‐γ‐iMSC‐EVs were isolated from the IFN‐γ‐iMSCs culture medium using ultracentrifugation. (e) Morphology of IFN‐γ‐iMSC‐EVs under cryo‐TEM. Scale bar = 200 nm. (f) Size distribution of IFN‐γ‐iMSC‐EVs shown by NTA. The average size is 134.6 nm. (g) Western blot analyses for markers of extracellular vesicles (CD9 and CD63) or cellular organelles (Cytochrome C and Histone H3) in IFN‐γ‐iMSCs and IFN‐γ‐iMSC‐EVs. (h) Expression analysis of EV markers (CD63 and CD81) in IFN‐γ‐iMSC‐EVs by flow cytometry.

    Article Snippet: To verify the expression of typical MSC surface markers on IFN‐γ‐primed iMSCs (IFN‐γ‐iMSCs), they were stained with antibodies against CD73 APC, CD105 PE, CD45 FITC and CD34 APC (eBioscience, Waltham, MA, USA), as well as CD90 APC‐Cy7 (BioLegend, San Diego, CA, USA).

    Techniques: Flow Cytometry, Expressing, Immunocytochemistry, Staining, Western Blot, Control, Isolation